PHASE 1. Monitoring the use and resistance to antimicrobial preparations
The stage of monitoring the use and resistance to antimicrobial preparations, involved the articulation and start of activities in all component projects. The activities carried out in the first stage, corresponding to 2018, have included:
For project 1, the construction of a i) database with “on line” access and ii) the adaptation of the classical microbiological methods (macrodilution, microdilution, diffusion around the discs) for screening bacteria (microbiological cut-off). Together with partner 3, Babes Bolyai University of Cluj Napoca, the following were achieved: iii) designing a questionnaire for the study of social perception on the use and resistance to antimicrobial products and iv) constructing a sample for the sociological study associated with the theme of the project (sociological cut-off).
In project 2, project managed by partner 1, Timisoara County Clinical Hospital through the research division (Oncogen Research Center), the activities were oriented towards i) investigations regarding hospital bacterial resistance, ii) bacterial resistance in animals with zoonotic potential, also involving iii) hypothesis formulation, extrapolation of strategies and methods. From a practical point of view, the project has supported iv) the establishment of an ″evidence bank″ with the establishment of dedicated cultures under standardized, reproducible conditions and was finalized with a study on distribution, population, biochemistry and resistance to antimicrobial agents.
Project 3, managed by the coordinator – University of Agricultural Sciences and Veterinary Medicine of Banat ″King Mihai I of Romania″ from Timisoara, Partner 1 (Oncogen Research Center) and Partner 4 (Research and Development Institute for Meadows, Brasov), addressed i) antimicrobial residues in the animal body, ii) study on the resistance of animals to infections as well as iii) the development of a pilot management program of mammary gland infections in order to reduce the use of antimicrobial preparations.
Project 4, managed in a narrower formula, by the Coordinator and Partner 1, involved: i) activities for the study of residues and antimicrobial preparations in food, feed and the environment, ii) identification of potentially resistant bacterial strains and characterization of the resistance mechanism. At the same time, the study was extended to iii) the biology of plants from the perspective of their potential to be used and on iv) the methods of cold-plasma sterilization.
Project 5, managed by Partner 2 (West University of Timisoara) and the Coordinator had as main objective the approach of the economic criteria regarding the quickly methods for cut-off of AMR, ii) building a data collection aiming to develop of iii) study models and iv) the study of operational cost.
Through the volume of activities, the results obtained and the result indicators, the first stage was completed according to the planning.
The activities of the five projects were carried out according to the work plan and can be quantified by the following indicators:
Articles (2)
1. Cristina Romeo Teodor, Moche J. P. Moche, Tyden Tyden, E., Hellmann Hellmann, K., Vendrig Vendrig JC, Şenel Şenel S, Dencker Dencker L, Linden Linden H, Schmerold Schmerold I, 2018, Network on veterinary medicines initiated by the European Federation For Pharmaceutical Sciences, Journal of Veterinary Pharmacology and Therapeutics.
2. Cristina Romeo Teodor, Doma Alexandru Octavian, Dumitrescu Eugenia, Muselin Florin, Andreia Chirila, 2018, Despre evoluția și implicațiile fenomenului rezistenței la medicamentele antiinfecțioase și antiparazitare de uz veterinar și evoluția acestui fenomen în România, Medicamentul Veterinar / Veterinary Drug
Paricipation to conferences (2)
1. Congress "Life Sciences – a Challenge for Future", Identification of pathogenic E.coli strains by DNA based molecular methods, Procedure for fast and accurate detection of antibiotic resistant E.Coli contamination in catle farms. Identification of pathogenic E.coli strains by DNA based molecular methods, Scientific papers. Veterinary Medicine Series" (ISSN 1454-7406)
2. 20th DKMT Conference on environment and health, Conference on environment and health, Boldura, Oana Maria, Procedure for fast and accurate detection of antibiotic resistant E.Coli contamination in catle farms
Services (5)
1. Services – bacteriological examination (identification, typing of bacteria and antibiograms)
2. Services of qualitative and quantitative analysis of milk
3. Services – qualitative and quantitative examination of feed
4. Services – veterinary clinical laboratory analysis
5. Services for early detection, diagnosis and treatment of mastitis at cows
Studies (17)
1. Samples and sampling methodology in AMR (Csaba, Degi, Iovu, Mihai Bogdan, Haragus, Paul Teodor)
6. Report on samples bank (Tanasie , Gabriela, Zambori, Csilla , Anghel , Simona , Tatu Carmen)
11. Study on operational costs (Iancu Tiberiu, Adamov Tabita Cornelia, Feher Andrea Ana, Hutu Ioan)
12. Report on tested procedures - quickly metods for cut-off of AMR (Chiș Codruta, Hutu Ioan, Emeric Ioan Toth)
17. Study on the plants biology (Imbrea Ilinca Merima, Alexa Ersilia Calina)
Other results (2)
